Construct: shRNA TRCN0000183146

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_020337.1-5458s1c1
DNA Barcode:
CCCTGAATAATTCATTGACTA

Original Target:

Taxon:
Homo sapiens (human)
Gene:
ANKRD50 (57182)
Gene Description:
ankyrin repeat domain 50
Transcript:
RefSeq NM_020337.1 (NON-CURRENT)
Match location:
Position 5458 (3UTR)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000183146)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CCCTGAATAATTCATTGACTA
Hairpin Sequence:
5'-CCGG-CCCTGAATAATTCATTGACTA-CTCGAG-TAGTCAATGAATTATTCAGGG-TTTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCCCTGAATAATTCATTGACTACTCGAGTAGTCAATGAATTATTCAGGGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACCCTGAATAATTCATTGACTACTCGAGTAGTCAATGAATTATTCAGGG-3'

Other clones with same target sequence:

(none)