Construct: shRNA TRCN0000183177

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_001007535.1-68s1c1
DNA Barcode:
GTGGAGAAGATGTTCATCTTT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
LOC286453 (286453)
Gene Description:
chondroitin sulfate N-acetylgalactosaminyltransferase 2 pseudogene
Transcript:
RefSeq NM_001007535.1 (NON-CURRENT)
Match location:
Position 68 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000183177)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GTGGAGAAGATGTTCATCTTT
Hairpin Sequence:
5'-CCGG-GTGGAGAAGATGTTCATCTTT-CTCGAG-AAAGATGAACATCTTCTCCAC-TTTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGTGGAGAAGATGTTCATCTTTCTCGAGAAAGATGAACATCTTCTCCACTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGTGGAGAAGATGTTCATCTTTCTCGAGAAAGATGAACATCTTCTCCAC-3'

Other clones with same target sequence:

(none)