Construct: shRNA TRCN0000183207

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_026592.2-493s1c1
DNA Barcode:
GACAACTTCCTAGACTTAGAA

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
B230118H07Rik (68170)
Gene Description:
RIKEN cDNA B230118H07 gene
Transcript:
RefSeq NM_026592.2 (NON-CURRENT)
Match location:
Position 493 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000183207)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GACAACTTCCTAGACTTAGAA
Hairpin Sequence:
5'-CCGG-GACAACTTCCTAGACTTAGAA-CTCGAG-TTCTAAGTCTAGGAAGTTGTC-TTTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGACAACTTCCTAGACTTAGAACTCGAGTTCTAAGTCTAGGAAGTTGTCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGACAACTTCCTAGACTTAGAACTCGAGTTCTAAGTCTAGGAAGTTGTC-3'

Other clones with same target sequence:

  1. TRCN0000339893