Construct: shRNA TRCN0000183218

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_001012324.1-451s1c1
DNA Barcode:
GAATCGCTTCTAAGTAATCTT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Ecm2 (407800)
Gene Description:
extracellular matrix protein 2, female organ and adipocyte specific
Transcript:
RefSeq NM_001012324.1 (NON-CURRENT)
Match location:
Position 451 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000183218)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GAATCGCTTCTAAGTAATCTT
Hairpin Sequence:
5'-CCGG-GAATCGCTTCTAAGTAATCTT-CTCGAG-AAGATTACTTAGAAGCGATTC-TTTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGAATCGCTTCTAAGTAATCTTCTCGAGAAGATTACTTAGAAGCGATTCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGAATCGCTTCTAAGTAATCTTCTCGAGAAGATTACTTAGAAGCGATTC-3'

Other clones with same target sequence:

(none)