Construct: shRNA TRCN0000183256

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_008528.3-1279s1c1
DNA Barcode:
GCATCAAATGTTTGTGAAGAA

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Blnk (17060)
Gene Description:
B cell linker
Transcript:
RefSeq NM_008528.3 (NON-CURRENT)
Match location:
Position 1279 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000183256)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCATCAAATGTTTGTGAAGAA
Hairpin Sequence:
5'-CCGG-GCATCAAATGTTTGTGAAGAA-CTCGAG-TTCTTCACAAACATTTGATGC-TTTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCATCAAATGTTTGTGAAGAACTCGAGTTCTTCACAAACATTTGATGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCATCAAATGTTTGTGAAGAACTCGAGTTCTTCACAAACATTTGATGC-3'

Other clones with same target sequence:

  1. TRCN0000329210