Construct: shRNA TRCN0000183301

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_014935.2-7058s1c1
DNA Barcode:
GCCAATTTGATTTGCTAGTAT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
PLEKHA6 (22874)
Gene Description:
pleckstrin homology domain containing A6
Transcript:
RefSeq NM_014935.2 (NON-CURRENT)
Match location:
Position 7058 (3UTR)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000183301)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCCAATTTGATTTGCTAGTAT
Hairpin Sequence:
5'-CCGG-GCCAATTTGATTTGCTAGTAT-CTCGAG-ATACTAGCAAATCAAATTGGC-TTTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCCAATTTGATTTGCTAGTATCTCGAGATACTAGCAAATCAAATTGGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCCAATTTGATTTGCTAGTATCTCGAGATACTAGCAAATCAAATTGGC-3'

Other clones with same target sequence:

(none)