Construct: shRNA TRCN0000183384

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_153138.2-1777s1c1
DNA Barcode:
GCCTAGCTGTTTATGTTGTAT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Wipf1 (215280)
Gene Description:
WAS/WASL interacting protein family, member 1
Transcript:
RefSeq NM_153138.2 (NON-CURRENT)
Match location:
Position 1777 (3UTR)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000183384)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCCTAGCTGTTTATGTTGTAT
Hairpin Sequence:
5'-CCGG-GCCTAGCTGTTTATGTTGTAT-CTCGAG-ATACAACATAAACAGCTAGGC-TTTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCCTAGCTGTTTATGTTGTATCTCGAGATACAACATAAACAGCTAGGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCCTAGCTGTTTATGTTGTATCTCGAGATACAACATAAACAGCTAGGC-3'

Other clones with same target sequence:

(none)