Construct: shRNA TRCN0000183431

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_173756.2-604s1c1
DNA Barcode:
CCTGTCATTCTGAAAGGTATT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Lin52 (217708)
Gene Description:
lin-52 homolog (C. elegans)
Transcript:
RefSeq NM_173756.2 (NON-CURRENT)
Match location:
Position 604 (3UTR)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000183431)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CCTGTCATTCTGAAAGGTATT
Hairpin Sequence:
5'-CCGG-CCTGTCATTCTGAAAGGTATT-CTCGAG-AATACCTTTCAGAATGACAGG-TTTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCCTGTCATTCTGAAAGGTATTCTCGAGAATACCTTTCAGAATGACAGGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACCTGTCATTCTGAAAGGTATTCTCGAGAATACCTTTCAGAATGACAGG-3'

Other clones with same target sequence:

(none)