Construct: shRNA TRCN0000183445

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_030888.2-1711s1c1
DNA Barcode:
GCTGATATAGTACAGAAGTTA

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
C1qtnf3 (81799)
Gene Description:
C1q and tumor necrosis factor related protein 3
Transcript:
RefSeq NM_030888.2 (NON-CURRENT)
Match location:
Position 1711 (3UTR)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000183445)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCTGATATAGTACAGAAGTTA
Hairpin Sequence:
5'-CCGG-GCTGATATAGTACAGAAGTTA-CTCGAG-TAACTTCTGTACTATATCAGC-TTTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCTGATATAGTACAGAAGTTACTCGAGTAACTTCTGTACTATATCAGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCTGATATAGTACAGAAGTTACTCGAGTAACTTCTGTACTATATCAGC-3'

Other clones with same target sequence:

(none)