Construct: shRNA TRCN0000183452

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_172964.1-1854s1c1
DNA Barcode:
GAAGGTTCCATCTTTCTTGAT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Arhgap28 (268970)
Gene Description:
Rho GTPase activating protein 28
Transcript:
RefSeq NM_172964.1 (NON-CURRENT)
Match location:
Position 1854 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000183452)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GAAGGTTCCATCTTTCTTGAT
Hairpin Sequence:
5'-CCGG-GAAGGTTCCATCTTTCTTGAT-CTCGAG-ATCAAGAAAGATGGAACCTTC-TTTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGAAGGTTCCATCTTTCTTGATCTCGAGATCAAGAAAGATGGAACCTTCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGAAGGTTCCATCTTTCTTGATCTCGAGATCAAGAAAGATGGAACCTTC-3'

Other clones with same target sequence:

(none)