Construct: shRNA TRCN0000183462
Construct Description:
- Construct Type:
- shRNA
- Other Identifiers:
- NM_019496.2-1728s1c1
- DNA Barcode:
- GCCTCATGTTATAGAACAATT
Original Target:
- Taxon:
-
Mus musculus (mouse)
- Gene:
- Ammecr1 (56068)
- Gene Description:
- Alport syndrome, mental retardation, midface hypoplasia and elliptocytosis chromosomal region gene 1
- Transcript:
- RefSeq NM_019496.2 (NON-CURRENT)
- Match location:
- Position 1728 (3UTR)
Vector Information:
- Vector Backbone:
- pLKO.1
- Pol II Cassette 1:
- PGK-PuroR
- Pol II Cassette 2:
- n/a
- Pol III Promoter:
- constitutive hU6
- Pol III Insert:
- (TRCN0000183462)
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
Current transcripts matched by this shRNA:
Sequence Information
- Target Sequence:
- GCCTCATGTTATAGAACAATT
- Hairpin Sequence:
-
5'-CCGG-GCCTCATGTTATAGAACAATT-CTCGAG-AATTGTTCTATAACATGAGGC-TTTTTTG-3'
Oligo design for arrayed cloning:
- Forward sequence:
- 5'-CCGGGCCTCATGTTATAGAACAATTCTCGAGAATTGTTCTATAACATGAGGCTTTTTG-3'
- Reverse sequence:
- 5'-AATTCAAAAAGCCTCATGTTATAGAACAATTCTCGAGAATTGTTCTATAACATGAGGC-3'
Other clones with same target sequence:
(none)
