Construct: shRNA TRCN0000183462

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_019496.2-1728s1c1
DNA Barcode:
GCCTCATGTTATAGAACAATT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Ammecr1 (56068)
Gene Description:
Alport syndrome, mental retardation, midface hypoplasia and elliptocytosis chromosomal region gene 1
Transcript:
RefSeq NM_019496.2 (NON-CURRENT)
Match location:
Position 1728 (3UTR)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000183462)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCCTCATGTTATAGAACAATT
Hairpin Sequence:
5'-CCGG-GCCTCATGTTATAGAACAATT-CTCGAG-AATTGTTCTATAACATGAGGC-TTTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCCTCATGTTATAGAACAATTCTCGAGAATTGTTCTATAACATGAGGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCCTCATGTTATAGAACAATTCTCGAGAATTGTTCTATAACATGAGGC-3'

Other clones with same target sequence:

(none)