Construct: shRNA TRCN0000183469

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_153584.1-3578s1c1
DNA Barcode:
GTACTGTTGAAACCACTTCAT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Fam214a (235493)
Gene Description:
family with sequence similarity 214, member A
Transcript:
RefSeq NM_153584.1 (NON-CURRENT)
Match location:
Position 3578 (3UTR)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000183469)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GTACTGTTGAAACCACTTCAT
Hairpin Sequence:
5'-CCGG-GTACTGTTGAAACCACTTCAT-CTCGAG-ATGAAGTGGTTTCAACAGTAC-TTTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGTACTGTTGAAACCACTTCATCTCGAGATGAAGTGGTTTCAACAGTACTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGTACTGTTGAAACCACTTCATCTCGAGATGAAGTGGTTTCAACAGTAC-3'

Other clones with same target sequence:

(none)