Construct: shRNA TRCN0000183471

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_172585.1-495s1c1
DNA Barcode:
GTTTCAGAGCTAAACCCTAAT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Larp4b (217980)
Gene Description:
La ribonucleoprotein domain family, member 4B
Transcript:
RefSeq NM_172585.1 (NON-CURRENT)
Match location:
Position 495 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000183471)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GTTTCAGAGCTAAACCCTAAT
Hairpin Sequence:
5'-CCGG-GTTTCAGAGCTAAACCCTAAT-CTCGAG-ATTAGGGTTTAGCTCTGAAAC-TTTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGTTTCAGAGCTAAACCCTAATCTCGAGATTAGGGTTTAGCTCTGAAACTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGTTTCAGAGCTAAACCCTAATCTCGAGATTAGGGTTTAGCTCTGAAAC-3'

Other clones with same target sequence:

  1. TRCN0000292802