Construct: shRNA TRCN0000183493

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_016150.3-2217s1c1
DNA Barcode:
GCTGATTAGATACCTGAAATA

Original Target:

Taxon:
Homo sapiens (human)
Gene:
ASB2 (51676)
Gene Description:
ankyrin repeat and SOCS box containing 2
Transcript:
RefSeq NM_016150.3 (NON-CURRENT)
Match location:
Position 2217 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000183493)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCTGATTAGATACCTGAAATA
Hairpin Sequence:
5'-CCGG-GCTGATTAGATACCTGAAATA-CTCGAG-TATTTCAGGTATCTAATCAGC-TTTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCTGATTAGATACCTGAAATACTCGAGTATTTCAGGTATCTAATCAGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCTGATTAGATACCTGAAATACTCGAGTATTTCAGGTATCTAATCAGC-3'

Other clones with same target sequence:

(none)