Construct: shRNA TRCN0000183523

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_024254.2-1494s1c1
DNA Barcode:
GCTAATTTCTTCTGTTGGAAA

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Katnbl1 (72425)
Gene Description:
katanin p80 subunit B like 1
Transcript:
RefSeq NM_024254.2 (NON-CURRENT)
Match location:
Position 1494 (3UTR)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000183523)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCTAATTTCTTCTGTTGGAAA
Hairpin Sequence:
5'-CCGG-GCTAATTTCTTCTGTTGGAAA-CTCGAG-TTTCCAACAGAAGAAATTAGC-TTTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCTAATTTCTTCTGTTGGAAACTCGAGTTTCCAACAGAAGAAATTAGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCTAATTTCTTCTGTTGGAAACTCGAGTTTCCAACAGAAGAAATTAGC-3'

Other clones with same target sequence:

(none)