Construct: shRNA TRCN0000183542

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_176832.1-2800s1c1
DNA Barcode:
GCTTAGAAATAACTGGGCAAT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Spire1 (68166)
Gene Description:
spire homolog 1 (Drosophila)
Transcript:
RefSeq NM_176832.1 (NON-CURRENT)
Match location:
Position 2800 (3UTR)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000183542)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCTTAGAAATAACTGGGCAAT
Hairpin Sequence:
5'-CCGG-GCTTAGAAATAACTGGGCAAT-CTCGAG-ATTGCCCAGTTATTTCTAAGC-TTTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCTTAGAAATAACTGGGCAATCTCGAGATTGCCCAGTTATTTCTAAGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCTTAGAAATAACTGGGCAATCTCGAGATTGCCCAGTTATTTCTAAGC-3'

Other clones with same target sequence:

(none)