Construct: shRNA TRCN0000183589

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_011369.1-2062s1c1
DNA Barcode:
GTCTTAAGTATGCTGCTTATA

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Shcbp1 (20419)
Gene Description:
Shc SH2-domain binding protein 1
Transcript:
RefSeq NM_011369.1 (NON-CURRENT)
Match location:
Position 2062 (3UTR)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000183589)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GTCTTAAGTATGCTGCTTATA
Hairpin Sequence:
5'-CCGG-GTCTTAAGTATGCTGCTTATA-CTCGAG-TATAAGCAGCATACTTAAGAC-TTTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGTCTTAAGTATGCTGCTTATACTCGAGTATAAGCAGCATACTTAAGACTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGTCTTAAGTATGCTGCTTATACTCGAGTATAAGCAGCATACTTAAGAC-3'

Other clones with same target sequence:

(none)