Construct: shRNA TRCN0000183626

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_020927.1-2099s1c1
DNA Barcode:
GCTCCATATCTGTTTCAATTT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
VAT1L (57687)
Gene Description:
vesicle amine transport 1 like
Transcript:
RefSeq NM_020927.1 (NON-CURRENT)
Match location:
Position 2099 (3UTR)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000183626)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCTCCATATCTGTTTCAATTT
Hairpin Sequence:
5'-CCGG-GCTCCATATCTGTTTCAATTT-CTCGAG-AAATTGAAACAGATATGGAGC-TTTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCTCCATATCTGTTTCAATTTCTCGAGAAATTGAAACAGATATGGAGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCTCCATATCTGTTTCAATTTCTCGAGAAATTGAAACAGATATGGAGC-3'

Other clones with same target sequence:

(none)