Construct: shRNA TRCN0000183639

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_172550.2-614s1c1
DNA Barcode:
GATTATAATCTGGGAGACATT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Ybey (216119)
Gene Description:
ybeY metallopeptidase
Transcript:
RefSeq NM_172550.2 (NON-CURRENT)
Match location:
Position 614 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000183639)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GATTATAATCTGGGAGACATT
Hairpin Sequence:
5'-CCGG-GATTATAATCTGGGAGACATT-CTCGAG-AATGTCTCCCAGATTATAATC-TTTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGATTATAATCTGGGAGACATTCTCGAGAATGTCTCCCAGATTATAATCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGATTATAATCTGGGAGACATTCTCGAGAATGTCTCCCAGATTATAATC-3'

Other clones with same target sequence:

(none)