Construct: shRNA TRCN0000183643

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_177817.2-899s1c1
DNA Barcode:
GCACTGAAATTGTGTAGTATA

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Gm5089 (328479)
Gene Description:
predicted gene 5089
Transcript:
RefSeq NM_177817.2 (NON-CURRENT)
Match location:
Position 899 (3UTR)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000183643)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCACTGAAATTGTGTAGTATA
Hairpin Sequence:
5'-CCGG-GCACTGAAATTGTGTAGTATA-CTCGAG-TATACTACACAATTTCAGTGC-TTTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCACTGAAATTGTGTAGTATACTCGAGTATACTACACAATTTCAGTGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCACTGAAATTGTGTAGTATACTCGAGTATACTACACAATTTCAGTGC-3'

Other clones with same target sequence:

(none)