Construct: shRNA TRCN0000183849

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_001011552.1-3335s1c1
DNA Barcode:
CCCTCCAAACTGAAACTAATT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
SLC9A4 (389015)
Gene Description:
solute carrier family 9 member A4
Transcript:
RefSeq NM_001011552.1 (NON-CURRENT)
Match location:
Position 3335 (3UTR)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000183849)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CCCTCCAAACTGAAACTAATT
Hairpin Sequence:
5'-CCGG-CCCTCCAAACTGAAACTAATT-CTCGAG-AATTAGTTTCAGTTTGGAGGG-TTTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCCCTCCAAACTGAAACTAATTCTCGAGAATTAGTTTCAGTTTGGAGGGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACCCTCCAAACTGAAACTAATTCTCGAGAATTAGTTTCAGTTTGGAGGG-3'

Other clones with same target sequence:

(none)