Construct: shRNA TRCN0000183893

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_001024672.1-235s1c1
DNA Barcode:
CTTCAGCAATCTGCAGGAGTT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Methig1 (554292)
Gene Description:
methyltransferase hypoxia inducible domain containing 1
Transcript:
RefSeq NM_001024672.1 (NON-CURRENT)
Match location:
Position 235 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000183893)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CTTCAGCAATCTGCAGGAGTT
Hairpin Sequence:
5'-CCGG-CTTCAGCAATCTGCAGGAGTT-CTCGAG-AACTCCTGCAGATTGCTGAAG-TTTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCTTCAGCAATCTGCAGGAGTTCTCGAGAACTCCTGCAGATTGCTGAAGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACTTCAGCAATCTGCAGGAGTTCTCGAGAACTCCTGCAGATTGCTGAAG-3'

Other clones with same target sequence:

(none)