Construct: shRNA TRCN0000183962

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_177897.3-2440s1c1
DNA Barcode:
CGTCAGGATGTCATGGTTCAT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
B4galnt4 (330671)
Gene Description:
beta-1,4-N-acetyl-galactosaminyl transferase 4
Transcript:
RefSeq NM_177897.3 (CURRENT)
Match location:
Position 2440 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000183962)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CGTCAGGATGTCATGGTTCAT
Hairpin Sequence:
5'-CCGG-CGTCAGGATGTCATGGTTCAT-CTCGAG-ATGAACCATGACATCCTGACG-TTTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCGTCAGGATGTCATGGTTCATCTCGAGATGAACCATGACATCCTGACGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACGTCAGGATGTCATGGTTCATCTCGAGATGAACCATGACATCCTGACG-3'

Other clones with same target sequence:

(none)