Construct: shRNA TRCN0000183990

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_175360.2-370s1c1
DNA Barcode:
CCAACAAGTGCCAGGTACATA

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Stn1 (108689)
Gene Description:
STN1, CST complex subunit
Transcript:
RefSeq NM_175360.2 (CURRENT)
Match location:
Position 370 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000183990)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CCAACAAGTGCCAGGTACATA
Hairpin Sequence:
5'-CCGG-CCAACAAGTGCCAGGTACATA-CTCGAG-TATGTACCTGGCACTTGTTGG-TTTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCCAACAAGTGCCAGGTACATACTCGAGTATGTACCTGGCACTTGTTGGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACCAACAAGTGCCAGGTACATACTCGAGTATGTACCTGGCACTTGTTGG-3'

Other clones with same target sequence:

(none)