Construct: shRNA TRCN0000184037

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_026263.1-611s1c1
DNA Barcode:
CCACAGATCCCAATGACGATT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
4930519G04Rik (67593)
Gene Description:
RIKEN cDNA 4930519G04 gene
Transcript:
RefSeq NM_026263.1 (NON-CURRENT)
Match location:
Position 611 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000184037)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CCACAGATCCCAATGACGATT
Hairpin Sequence:
5'-CCGG-CCACAGATCCCAATGACGATT-CTCGAG-AATCGTCATTGGGATCTGTGG-TTTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCCACAGATCCCAATGACGATTCTCGAGAATCGTCATTGGGATCTGTGGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACCACAGATCCCAATGACGATTCTCGAGAATCGTCATTGGGATCTGTGG-3'

Other clones with same target sequence:

(none)