Construct: shRNA TRCN0000184101

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_027580.1-867s1c1
DNA Barcode:
CCCGCCATTGTTATAGCACTT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Vwa3b (70853)
Gene Description:
von Willebrand factor A domain containing 3B
Transcript:
RefSeq NM_027580.1 (NON-CURRENT)
Match location:
Position 867 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000184101)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CCCGCCATTGTTATAGCACTT
Hairpin Sequence:
5'-CCGG-CCCGCCATTGTTATAGCACTT-CTCGAG-AAGTGCTATAACAATGGCGGG-TTTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCCCGCCATTGTTATAGCACTTCTCGAGAAGTGCTATAACAATGGCGGGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACCCGCCATTGTTATAGCACTTCTCGAGAAGTGCTATAACAATGGCGGG-3'

Other clones with same target sequence:

(none)