Construct: shRNA TRCN0000184142

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_146110.1-1247s1c1
DNA Barcode:
CCTGCTTACTCCTTGTCCTTT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Aamp (227290)
Gene Description:
angio-associated migratory protein
Transcript:
RefSeq NM_146110.1 (NON-CURRENT)
Match location:
Position 1247 (3UTR)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000184142)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CCTGCTTACTCCTTGTCCTTT
Hairpin Sequence:
5'-CCGG-CCTGCTTACTCCTTGTCCTTT-CTCGAG-AAAGGACAAGGAGTAAGCAGG-TTTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCCTGCTTACTCCTTGTCCTTTCTCGAGAAAGGACAAGGAGTAAGCAGGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACCTGCTTACTCCTTGTCCTTTCTCGAGAAAGGACAAGGAGTAAGCAGG-3'

Other clones with same target sequence:

  1. TRCN0000314384