Construct: shRNA TRCN0000184158

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_025657.2-1319s1c1
DNA Barcode:
CCTTTGTCAAAGGAGCACCAA

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Lrrc57 (66606)
Gene Description:
leucine rich repeat containing 57
Transcript:
RefSeq NM_025657.2 (NON-CURRENT)
Match location:
Position 1319 (3UTR)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000184158)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CCTTTGTCAAAGGAGCACCAA
Hairpin Sequence:
5'-CCGG-CCTTTGTCAAAGGAGCACCAA-CTCGAG-TTGGTGCTCCTTTGACAAAGG-TTTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCCTTTGTCAAAGGAGCACCAACTCGAGTTGGTGCTCCTTTGACAAAGGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACCTTTGTCAAAGGAGCACCAACTCGAGTTGGTGCTCCTTTGACAAAGG-3'

Other clones with same target sequence:

(none)