Construct: shRNA TRCN0000184222

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_028381.1-527s1c1
DNA Barcode:
GCAAGAGCTGAAAGACCTGAA

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Ccdc94 (72886)
Gene Description:
coiled-coil domain containing 94
Transcript:
RefSeq NM_028381.1 (NON-CURRENT)
Match location:
Position 527 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000184222)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCAAGAGCTGAAAGACCTGAA
Hairpin Sequence:
5'-CCGG-GCAAGAGCTGAAAGACCTGAA-CTCGAG-TTCAGGTCTTTCAGCTCTTGC-TTTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCAAGAGCTGAAAGACCTGAACTCGAGTTCAGGTCTTTCAGCTCTTGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCAAGAGCTGAAAGACCTGAACTCGAGTTCAGGTCTTTCAGCTCTTGC-3'

Other clones with same target sequence:

(none)