Construct: shRNA TRCN0000184256

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_133348.1-493s1c1
DNA Barcode:
GCTGACCAATAAAGCCACCTT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Acot7 (70025)
Gene Description:
acyl-CoA thioesterase 7
Transcript:
RefSeq NM_133348.1 (NON-CURRENT)
Match location:
Position 493 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000184256)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCTGACCAATAAAGCCACCTT
Hairpin Sequence:
5'-CCGG-GCTGACCAATAAAGCCACCTT-CTCGAG-AAGGTGGCTTTATTGGTCAGC-TTTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCTGACCAATAAAGCCACCTTCTCGAGAAGGTGGCTTTATTGGTCAGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCTGACCAATAAAGCCACCTTCTCGAGAAGGTGGCTTTATTGGTCAGC-3'

Other clones with same target sequence:

  1. TRCN0000336539