Construct: shRNA TRCN0000184257

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_133734.1-388s1c1
DNA Barcode:
GATCAAGACACAGGTGGGATT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Dcaf11 (28199)
Gene Description:
DDB1 and CUL4 associated factor 11
Transcript:
RefSeq NM_133734.1 (NON-CURRENT)
Match location:
Position 388 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000184257)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GATCAAGACACAGGTGGGATT
Hairpin Sequence:
5'-CCGG-GATCAAGACACAGGTGGGATT-CTCGAG-AATCCCACCTGTGTCTTGATC-TTTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGATCAAGACACAGGTGGGATTCTCGAGAATCCCACCTGTGTCTTGATCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGATCAAGACACAGGTGGGATTCTCGAGAATCCCACCTGTGTCTTGATC-3'

Other clones with same target sequence:

  1. TRCN0000336187