Construct: shRNA TRCN0000184261

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_175360.2-434s1c1
DNA Barcode:
GCTGTCATCAGCGTGAAAGAA

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Stn1 (108689)
Gene Description:
STN1, CST complex subunit
Transcript:
RefSeq NM_175360.2 (CURRENT)
Match location:
Position 434 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000184261)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCTGTCATCAGCGTGAAAGAA
Hairpin Sequence:
5'-CCGG-GCTGTCATCAGCGTGAAAGAA-CTCGAG-TTCTTTCACGCTGATGACAGC-TTTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCTGTCATCAGCGTGAAAGAACTCGAGTTCTTTCACGCTGATGACAGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCTGTCATCAGCGTGAAAGAACTCGAGTTCTTTCACGCTGATGACAGC-3'

Other clones with same target sequence:

(none)