Construct: shRNA TRCN0000184270

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_009133.2-694s1c1
DNA Barcode:
GCTCAACTACAAGATGGAGCT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Stmn3 (20262)
Gene Description:
stathmin-like 3
Transcript:
RefSeq NM_009133.2 (NON-CURRENT)
Match location:
Position 694 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000184270)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCTCAACTACAAGATGGAGCT
Hairpin Sequence:
5'-CCGG-GCTCAACTACAAGATGGAGCT-CTCGAG-AGCTCCATCTTGTAGTTGAGC-TTTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCTCAACTACAAGATGGAGCTCTCGAGAGCTCCATCTTGTAGTTGAGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCTCAACTACAAGATGGAGCTCTCGAGAGCTCCATCTTGTAGTTGAGC-3'

Other clones with same target sequence:

(none)