Construct: shRNA TRCN0000184308

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_153424.1-418s1c1
DNA Barcode:
GCCACTCATTAAGCAGGGAAT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Nphp4 (260305)
Gene Description:
nephronophthisis 4 (juvenile) homolog (human)
Transcript:
RefSeq NM_153424.1 (NON-CURRENT)
Match location:
Position 418 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000184308)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCCACTCATTAAGCAGGGAAT
Hairpin Sequence:
5'-CCGG-GCCACTCATTAAGCAGGGAAT-CTCGAG-ATTCCCTGCTTAATGAGTGGC-TTTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCCACTCATTAAGCAGGGAATCTCGAGATTCCCTGCTTAATGAGTGGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCCACTCATTAAGCAGGGAATCTCGAGATTCCCTGCTTAATGAGTGGC-3'

Other clones with same target sequence:

(none)