Construct: shRNA TRCN0000184328

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_027650.1-279s1c1
DNA Barcode:
GATCGCCTGATCTCTTTCCAA

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Speer3 (71026)
Gene Description:
spermatogenesis associated glutamate (E)-rich protein 3
Transcript:
RefSeq NM_027650.1 (NON-CURRENT)
Match location:
Position 279 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000184328)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GATCGCCTGATCTCTTTCCAA
Hairpin Sequence:
5'-CCGG-GATCGCCTGATCTCTTTCCAA-CTCGAG-TTGGAAAGAGATCAGGCGATC-TTTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGATCGCCTGATCTCTTTCCAACTCGAGTTGGAAAGAGATCAGGCGATCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGATCGCCTGATCTCTTTCCAACTCGAGTTGGAAAGAGATCAGGCGATC-3'

Other clones with same target sequence:

(none)