Construct: shRNA TRCN0000184351

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_026732.2-373s1c1
DNA Barcode:
GTTTGACTCCAACAACGTGGT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Mrpl14 (68463)
Gene Description:
mitochondrial ribosomal protein L14
Transcript:
RefSeq NM_026732.2 (CURRENT)
Match location:
Position 373 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000184351)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GTTTGACTCCAACAACGTGGT
Hairpin Sequence:
5'-CCGG-GTTTGACTCCAACAACGTGGT-CTCGAG-ACCACGTTGTTGGAGTCAAAC-TTTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGTTTGACTCCAACAACGTGGTCTCGAGACCACGTTGTTGGAGTCAAACTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGTTTGACTCCAACAACGTGGTCTCGAGACCACGTTGTTGGAGTCAAAC-3'

Other clones with same target sequence:

  1. TRCN0000276862