Construct: shRNA TRCN0000184367

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_005947.1-205s1c1
DNA Barcode:
GCAAAGGCTCATCAGAGAAGT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
MT1B (4490)
Gene Description:
metallothionein 1B
Transcript:
RefSeq NM_005947.1 (NON-CURRENT)
Match location:
Position 205 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000184367)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCAAAGGCTCATCAGAGAAGT
Hairpin Sequence:
5'-CCGG-GCAAAGGCTCATCAGAGAAGT-CTCGAG-ACTTCTCTGATGAGCCTTTGC-TTTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCAAAGGCTCATCAGAGAAGTCTCGAGACTTCTCTGATGAGCCTTTGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCAAAGGCTCATCAGAGAAGTCTCGAGACTTCTCTGATGAGCCTTTGC-3'

Other clones with same target sequence:

(none)