Construct: shRNA TRCN0000184420

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_024166.4-565s1c1
DNA Barcode:
GACCTTGCTCTCTAGAGATCA

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Chchd2 (14004)
Gene Description:
coiled-coil-helix-coiled-coil-helix domain containing 2
Transcript:
RefSeq NM_024166.4 (NON-CURRENT)
Match location:
Position 565 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000184420)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GACCTTGCTCTCTAGAGATCA
Hairpin Sequence:
5'-CCGG-GACCTTGCTCTCTAGAGATCA-CTCGAG-TGATCTCTAGAGAGCAAGGTC-TTTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGACCTTGCTCTCTAGAGATCACTCGAGTGATCTCTAGAGAGCAAGGTCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGACCTTGCTCTCTAGAGATCACTCGAGTGATCTCTAGAGAGCAAGGTC-3'

Other clones with same target sequence:

(none)