Construct: shRNA TRCN0000184522

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_016743.1-1841s1c1
DNA Barcode:
GCGGAGAATCCTGTGAAGATA

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Nell2 (54003)
Gene Description:
NEL-like 2
Transcript:
RefSeq NM_016743.1 (NON-CURRENT)
Match location:
Position 1841 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000184522)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCGGAGAATCCTGTGAAGATA
Hairpin Sequence:
5'-CCGG-GCGGAGAATCCTGTGAAGATA-CTCGAG-TATCTTCACAGGATTCTCCGC-TTTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCGGAGAATCCTGTGAAGATACTCGAGTATCTTCACAGGATTCTCCGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCGGAGAATCCTGTGAAGATACTCGAGTATCTTCACAGGATTCTCCGC-3'

Other clones with same target sequence:

(none)