Construct: shRNA TRCN0000184540

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_011843.1-3545s1c1
DNA Barcode:
GCCTGTTACCTCTTCCTTCTT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Esyt1 (23943)
Gene Description:
extended synaptotagmin-like protein 1
Transcript:
RefSeq NM_011843.1 (NON-CURRENT)
Match location:
Position 3545 (3UTR)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000184540)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCCTGTTACCTCTTCCTTCTT
Hairpin Sequence:
5'-CCGG-GCCTGTTACCTCTTCCTTCTT-CTCGAG-AAGAAGGAAGAGGTAACAGGC-TTTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCCTGTTACCTCTTCCTTCTTCTCGAGAAGAAGGAAGAGGTAACAGGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCCTGTTACCTCTTCCTTCTTCTCGAGAAGAAGGAAGAGGTAACAGGC-3'

Other clones with same target sequence:

(none)