Construct: shRNA TRCN0000184541

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_013872.1-518s1c1
DNA Barcode:
GTTCTCGGAACTGGACAAGAA

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Pmm1 (29858)
Gene Description:
phosphomannomutase 1
Transcript:
RefSeq NM_013872.1 (NON-CURRENT)
Match location:
Position 518 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000184541)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GTTCTCGGAACTGGACAAGAA
Hairpin Sequence:
5'-CCGG-GTTCTCGGAACTGGACAAGAA-CTCGAG-TTCTTGTCCAGTTCCGAGAAC-TTTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGTTCTCGGAACTGGACAAGAACTCGAGTTCTTGTCCAGTTCCGAGAACTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGTTCTCGGAACTGGACAAGAACTCGAGTTCTTGTCCAGTTCCGAGAAC-3'

Other clones with same target sequence:

(none)