Construct: shRNA TRCN0000184603

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_172501.1-320s1c1
DNA Barcode:
GATTCCCTCAACGGGTTAGTT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Nhlrc3 (212114)
Gene Description:
NHL repeat containing 3
Transcript:
RefSeq NM_172501.1 (NON-CURRENT)
Match location:
Position 320 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000184603)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GATTCCCTCAACGGGTTAGTT
Hairpin Sequence:
5'-CCGG-GATTCCCTCAACGGGTTAGTT-CTCGAG-AACTAACCCGTTGAGGGAATC-TTTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGATTCCCTCAACGGGTTAGTTCTCGAGAACTAACCCGTTGAGGGAATCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGATTCCCTCAACGGGTTAGTTCTCGAGAACTAACCCGTTGAGGGAATC-3'

Other clones with same target sequence:

(none)