Construct: shRNA TRCN0000184604

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_175392.3-722s1c1
DNA Barcode:
GCAAGAGCAACGACACTCTTA

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Miga2 (108958)
Gene Description:
mitoguardin 2
Transcript:
RefSeq NM_175392.3 (CURRENT)
Match location:
Position 722 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000184604)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCAAGAGCAACGACACTCTTA
Hairpin Sequence:
5'-CCGG-GCAAGAGCAACGACACTCTTA-CTCGAG-TAAGAGTGTCGTTGCTCTTGC-TTTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCAAGAGCAACGACACTCTTACTCGAGTAAGAGTGTCGTTGCTCTTGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCAAGAGCAACGACACTCTTACTCGAGTAAGAGTGTCGTTGCTCTTGC-3'

Other clones with same target sequence:

(none)