Construct: shRNA TRCN0000184617

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_026937.1-1180s1c1
DNA Barcode:
GAGAACCATGACTGACTGGAT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Ascc1 (69090)
Gene Description:
activating signal cointegrator 1 complex subunit 1
Transcript:
RefSeq NM_026937.1 (NON-CURRENT)
Match location:
Position 1180 (3UTR)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000184617)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GAGAACCATGACTGACTGGAT
Hairpin Sequence:
5'-CCGG-GAGAACCATGACTGACTGGAT-CTCGAG-ATCCAGTCAGTCATGGTTCTC-TTTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGAGAACCATGACTGACTGGATCTCGAGATCCAGTCAGTCATGGTTCTCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGAGAACCATGACTGACTGGATCTCGAGATCCAGTCAGTCATGGTTCTC-3'

Other clones with same target sequence:

(none)