Construct: shRNA TRCN0000184635

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_026937.1-1311s1c1
DNA Barcode:
CCTTGGCTTCTGTCCTTCATT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Ascc1 (69090)
Gene Description:
activating signal cointegrator 1 complex subunit 1
Transcript:
RefSeq NM_026937.1 (NON-CURRENT)
Match location:
Position 1311 (3UTR)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000184635)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CCTTGGCTTCTGTCCTTCATT
Hairpin Sequence:
5'-CCGG-CCTTGGCTTCTGTCCTTCATT-CTCGAG-AATGAAGGACAGAAGCCAAGG-TTTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCCTTGGCTTCTGTCCTTCATTCTCGAGAATGAAGGACAGAAGCCAAGGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACCTTGGCTTCTGTCCTTCATTCTCGAGAATGAAGGACAGAAGCCAAGG-3'

Other clones with same target sequence:

(none)