Construct: shRNA TRCN0000184700

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_018660.2-576s1c1
DNA Barcode:
CAGGAACATCGATGTCCCAAA

Original Target:

Taxon:
Homo sapiens (human)
Gene:
ZNF395 (55893)
Gene Description:
zinc finger protein 395
Transcript:
RefSeq NM_018660.2 (NON-CURRENT)
Match location:
Position 576 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000184700)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CAGGAACATCGATGTCCCAAA
Hairpin Sequence:
5'-CCGG-CAGGAACATCGATGTCCCAAA-CTCGAG-TTTGGGACATCGATGTTCCTG-TTTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCAGGAACATCGATGTCCCAAACTCGAGTTTGGGACATCGATGTTCCTGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACAGGAACATCGATGTCCCAAACTCGAGTTTGGGACATCGATGTTCCTG-3'

Other clones with same target sequence:

(none)