Construct: shRNA TRCN0000184703

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_025526.1-665s1c1
DNA Barcode:
CGTGTGTCCTTAGTGCAGTTA

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Iscu (66383)
Gene Description:
iron-sulfur cluster assembly enzyme
Transcript:
RefSeq NM_025526.1 (NON-CURRENT)
Match location:
Position 665 (3UTR)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000184703)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CGTGTGTCCTTAGTGCAGTTA
Hairpin Sequence:
5'-CCGG-CGTGTGTCCTTAGTGCAGTTA-CTCGAG-TAACTGCACTAAGGACACACG-TTTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCGTGTGTCCTTAGTGCAGTTACTCGAGTAACTGCACTAAGGACACACGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACGTGTGTCCTTAGTGCAGTTACTCGAGTAACTGCACTAAGGACACACG-3'

Other clones with same target sequence:

  1. TRCN0000353442