Construct: shRNA TRCN0000184785

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_172500.1-4843s1c1
DNA Barcode:
CCTGAGTTCCATGACCTGAAT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Syne3 (212073)
Gene Description:
spectrin repeat containing, nuclear envelope family member 3
Transcript:
RefSeq NM_172500.1 (NON-CURRENT)
Match location:
Position 4843 (3UTR)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000184785)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CCTGAGTTCCATGACCTGAAT
Hairpin Sequence:
5'-CCGG-CCTGAGTTCCATGACCTGAAT-CTCGAG-ATTCAGGTCATGGAACTCAGG-TTTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCCTGAGTTCCATGACCTGAATCTCGAGATTCAGGTCATGGAACTCAGGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACCTGAGTTCCATGACCTGAATCTCGAGATTCAGGTCATGGAACTCAGG-3'

Other clones with same target sequence:

(none)