Construct: shRNA TRCN0000184968

Construct Description:

Construct Type:
shRNA
Other Identifiers:
XM_069595.4-475s1c1
DNA Barcode:
CAACTGGCATTTCTTGTTAAT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
OR4F16 (135896)
Gene Description:
olfactory receptor OR4F16
Transcript:
RefSeq XM_069595.4 (NON-CURRENT)
Match location:
Position 475 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000184968)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CAACTGGCATTTCTTGTTAAT
Hairpin Sequence:
5'-CCGG-CAACTGGCATTTCTTGTTAAT-CTCGAG-ATTAACAAGAAATGCCAGTTG-TTTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCAACTGGCATTTCTTGTTAATCTCGAGATTAACAAGAAATGCCAGTTGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACAACTGGCATTTCTTGTTAATCTCGAGATTAACAAGAAATGCCAGTTG-3'

Other clones with same target sequence:

(none)