Construct: shRNA TRCN0000185209

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_026954.1-950s1c1
DNA Barcode:
CAACTCTAAGACCTGCTTTAA

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Tusc1 (69136)
Gene Description:
tumor suppressor candidate 1
Transcript:
RefSeq NM_026954.1 (CURRENT)
Match location:
Position 950 (3UTR)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000185209)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CAACTCTAAGACCTGCTTTAA
Hairpin Sequence:
5'-CCGG-CAACTCTAAGACCTGCTTTAA-CTCGAG-TTAAAGCAGGTCTTAGAGTTG-TTTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCAACTCTAAGACCTGCTTTAACTCGAGTTAAAGCAGGTCTTAGAGTTGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACAACTCTAAGACCTGCTTTAACTCGAGTTAAAGCAGGTCTTAGAGTTG-3'

Other clones with same target sequence:

  1. TRCN0000254480